Real Time In Vivo Evaluation of Mitochondrial Activity and Brain Functions during Ischemic Stroke

More than 50% of total energy consumed by the brain is utilized active transport processes which are responsible for keeping ionic homeostasis in brain. Under an ischemic condition, availability limited, and, as a result, inhibition ion pumps unavoidable. The initial consequence such gradual accumulation K+ extracellular space leading to second phase depolarization phenomenon. During depolarization, will increase 15-20-fold, while Ca2+ decreasing 10 fold. Another optional effect mild ischemia development Cortical Spreading Depression due leakage into space. Mongolian gerbil provides very useful animal model study effects on functions. aims were follows: (1) To elucidate mechanism behind or cortical spreading depression under unilateral and bilateral carotid artery occlusion. (2) correlate kinetics recovery level ischemia. We tested correlation between depletion (evaluated intramitochondrial NADH redox state Cerebral Blood Flow) K+, H+, Ca2+, DC potential 366 nm reflectance changes) partial complete using multiparametric monitoring system. results could be summarized occlusion, all gerbils was recorded within 1-2 min. obtained significantly smaller led about 60% gerbils. (3) during had 'all none' nature terms maximal K÷ levels time reach it. (4) main various lengths occlusion level. (5) develop most cases from event when not episode.